Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jan 23;166(1):67-70.
doi: 10.1016/0014-5793(84)80046-0.

Expression of human alpha 1-antitrypsin in Escherichia coli

A Bollen, R Loriau, A Herzog, P Hérion
Free article

Expression of human alpha 1-antitrypsin in Escherichia coli

A Bollen et al. FEBS Lett. .
Free article

Abstract

Complementary DNA coding for human alpha 1-antitrypsin has been placed under the control of the lambda PR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human alpha 1-antitrypsin (+/- 9000 molecules/cell). The product has a Mr of 44000, corresponding to mature unglycosylated alpha 1-antitrypsin.

PubMed Disclaimer

Publication types

LinkOut - more resources