The use of antisera and monoclonal antibodies to identify the antigen-specific T cell receptor from pigeon cytochrome c-specific T cell hybrids

Abstract

Pigeon cytochrome c-specific, Ek beta:Ek alpha Ia molecule-restricted T cell hybrids were used as immunogens in order to obtain antisera and monoclonal antibodies directed against the antigen receptor. Antisera against 2 different T cell hybrids specifically altered the IL 2 release only from the immunizing clone. Monoclonal antibodies against one of these hybrids were also obtained. They specifically bound to and inhibited the IL 2 release only from the immunizing cell. Lectin-induced IL 2 release was not blocked by these monoclonals. Immunoprecipitation and SDS-PAGE analysis of detergent lysates from surface-labelled hybrid cells demonstrated a heterodimeric structure composed of chains of 45-50 and 40-44 kd apparent molecular weight. These chains were linked by disulfide bonds and each appeared to contain intramolecular disulfide bonds as well. The molecule that has been isolated is likely to be the receptor for antigen on the immunizing clone.