Physical organization of the metJB component of the Escherichia coli K-12 metJBLF gene cluster

Abstract

The structures of a series of plaque-forming metJB transducing phage were studied by restriction endonuclease mapping and enzyme activity assay. One of these phage, lambda pmet100, was inactivated by heat shock in the presence of EDTA, and deletion mutants were selected from the survivors. Two of these mutants, lambda pmet100 delta 1 and lambda pmet100 delta 2, were used to confirm the gene order metJ metB when moving clockwise on the linkage map of Escherichia coli K-12. Additional results indicate that the metB gene can be expressed independently of any other component of the met regulon and that the metJ gene also forms a separate transcription unit.