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Comparative Study
. 1984 Feb 10;259(3):1375-7.

Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity

  • PMID: 6319395
Free article
Comparative Study

Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity

C Thrash et al. J Biol Chem. .
Free article

Abstract

Mutants of the yeast, Saccharomyces cerevisiae, deficient in DNA topoisomerase I activity have been identified. One mutant has normal topoisomerase I activity when assayed at 25 degrees C and about 20% of normal activity when assayed at 36 degrees C. Strains with this mutation grow normally at all temperatures tested. The mutation has been mapped to MAK1, a gene required for maintenance of killer RNA. Three previously isolated mak1 mutants exhibit less than 1% of normal topoisomerase I activity in our assay, but yet they grow normally. The implications of these results for the role of DNA topoisomerase I in the cell are discussed.

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