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Comparative Study
. 1984 Jan 25;12(2):887-99.
doi: 10.1093/nar/12.2.887.

The nucleotide recognized by the Escherichia coli A restriction and modification enzyme

M Kröger, G Hobom
Free PMC article
Comparative Study

The nucleotide recognized by the Escherichia coli A restriction and modification enzyme

M Kröger et al. Nucleic Acids Res. .
Free PMC article

Abstract

The nucleotide recognition sequence for the restriction-modification enzyme of Escherichia coli A (EcoA) has been determined to be GAG-7N-GTCA. This sequence is fairly similar, but distinctly different from the two other type I restriction enzyme recognition sites known for E. coli B and E. coli K12, respectively. N6-adenosine methylation has been observed at nucleotide positions 2 and 12 within that sequence after modification by EcoA. As a reference point for mapping the single EcoA site in lambda, the position of lambda point mutation Oam29 has been determined also.

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