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. 1984 Mar;36(3):593-8.
doi: 10.1016/0092-8674(84)90338-6.

Synapsis promoted by Ustilago rec1 protein

E B Kmiec, W K Holloman

Synapsis promoted by Ustilago rec1 protein

E B Kmiec et al. Cell. 1984 Mar.

Abstract

Ustilago rec 1 protein pairs homologous DNA molecules by promoting both synapsis and strand transfer. Complexes formed with rec 1 protein and a homologous combination of single-stranded and duplex DNA that appear to be synaptic structures can be detected by use of a nitrocellulose filter assay. The nascent heteroduplex formed during synapsis is a paranemic joint in which the single-stranded DNA pairs, but does not interwind, with its complement in the duplex molecule. Formation of the paranemic joint is accompanied by duplex unwinding and genesis of left-handed Z-DNA.

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