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. 1984 Feb;19(2):112-5.
doi: 10.1128/jcm.19.2.112-115.1984.

Rapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay

B A McClane, R J Strouse

Rapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay

B A McClane et al. J Clin Microbiol. 1984 Feb.

Abstract

Clostridium perfringens type A enterotoxin was specifically detected and readily quantified by indirect and four-layer sandwich enzyme-linked immunosorbent assays (ELISAs). With the indirect ELISA, enterotoxin was detected in quantities of as low as 2.5 ng (25 ng/ml). When the more sensitive sandwich ELISA procedures was used, 100 pg (1 ng/ml) of enterotoxin was detected. The sandwich ELISA procedure specifically detected enterotoxin in human fecal extracts. Additionally, the sandwich ELISA specifically differentiated enterotoxin-positive strains from enterotoxin-negative strains of C. perfringens. Both the indirect and sandwich ELISA procedures described for C. perfringens enterotoxin in this report are rapid, specific, sensitive, and easily adaptable for large-scale use by clinical or research laboratories.

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