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. 1984 Feb;81(3):669-73.
doi: 10.1073/pnas.81.3.669.

High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli

M Courtney, A Buchwalder, L H Tessier, M Jaye, A Benavente, A Balland, V Kohli, R Lathe, P Tolstoshev, J P Lecocq

High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli

M Courtney et al. Proc Natl Acad Sci U S A. 1984 Feb.

Abstract

A cDNA clone containing the complete human alpha 1-antitrypsin sequence was isolated from a human liver cDNA bank by screening with a chemically synthesized oligonucleotide probe. DNA sequences encoding the alpha 1-antitrypsin mature polypeptide were inserted into an Escherichia coli expression vector that allows transcription from the efficient leftward promoter of bacteriophage lambda (PL) and initiation of translation at the lambda cII gene ribosome-binding site. This construction resulted in the induction of a 45-kilodalton protein at a level of approximately 15% of total cell protein. The polypeptide produced was recognized by antisera raised against human alpha 1-antitrypsin protein and displayed normal biological activity in an in vitro antielastase assay.

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References

    1. EMBO J. 1983;2(1):57-61 - PubMed
    1. DNA. 1983;2(4):255-64 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Nature. 1975 Mar 6;254(5495):34-8 - PubMed
    1. N Engl J Med. 1975 Sep 18;293(12):576-9 - PubMed

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