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. 1984 Feb;81(4):1070-4.
doi: 10.1073/pnas.81.4.1070.

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells

T Yokota, F Lee, D Rennick, C Hall, N Arai, T Mosmann, G Nabel, H Cantor, K Arai

Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells

T Yokota et al. Proc Natl Acad Sci U S A. 1984 Feb.

Abstract

A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a cDNA library prepared from mRNA derived from a concanavalin A-activated mouse T-cell clone. Cloning was achieved using the pcD vector that permits expression of cDNA inserts in mammalian cells. The DNA sequence codes for a polypeptide of 166 amino acid residues including a putative signal peptide. The supernatant fluid obtained after transfection of COS-7 monkey cells with the pcD-MCGF plasmid had mouse MCGF activity. The MCGF amino acid sequence between amino acids 33 and 41, deduced from the nucleotide sequence of its cDNA, is identical to the NH2-terminal sequence of the mouse lymphokine, interleukin 3, suggesting that MCGF is related to interleukin 3.

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References

    1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 - PubMed
    1. J Immunol Methods. 1983 Dec 16;65(1-2):55-63 - PubMed
    1. J Exp Med. 1975 Jun 1;141(6):1390-9 - PubMed
    1. Biochemistry. 1977 Oct 18;16(21):4743-51 - PubMed
    1. J Exp Med. 1978 Feb 1;147(2):324-39 - PubMed

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