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. 1984 Feb 14;118(3):747-52.
doi: 10.1016/0006-291x(84)91458-x.

Negatively supercoiled DNA from plants infected with a single-stranded DNA virus

G Sunter, R H Coutts, K W Buck

Negatively supercoiled DNA from plants infected with a single-stranded DNA virus

G Sunter et al. Biochem Biophys Res Commun. .

Abstract

A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of -0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme.

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