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Comparative Study
. 1983 Dec;26(2-3):295-9.
doi: 10.1016/0378-1119(83)90200-7.

Expression in Streptomyces ambofaciens of an Escherichia coli K-12 gene which confers resistance to hygromycin B

S Kuhstoss, R N Rao
Comparative Study

Expression in Streptomyces ambofaciens of an Escherichia coli K-12 gene which confers resistance to hygromycin B

S Kuhstoss et al. Gene. 1983 Dec.

Abstract

We have constructed two plasmid vectors (pKC293 and pKC305) that can replicate in Escherichia coli K-12 and Streptomyces ambofaciens. These shuttle vectors were used to demonstrate the expression of two E. coli genes, hygromycin B (Hm) resistance and Tn5 neomycin (Nm) resistance, in S. ambofaciens.

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