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. 1984 May;50(2):645-8.
doi: 10.1128/JVI.50.2.645-648.1984.

Identification and genetic mapping of a herpes simplex virus capsid protein that binds DNA

D K Braun, W Batterson, B Roizman

Identification and genetic mapping of a herpes simplex virus capsid protein that binds DNA

D K Braun et al. J Virol. 1984 May.

Abstract

Herpes simplex virus virion protein 19C (VP19C) is a constituent of both unenveloped (nuclear) and enveloped (cytoplasmic) capsids. In this paper we report that 32P-labeled DNA, either supercoiled or linear double stranded, efficiently bound to VP19C electrically transferred from denaturing polyacrylamide gels containing electrophoretically separated proteins from purified capsids. Analyses of the polypeptides specified by herpes simplex virus type 1 X herpes simplex type 2 recombinants with respect to electrophoretic mobility and binding of 32P-labeled DNA indicate that VP19C maps at the same location as infected cell polypeptide 32 and is derived from it.

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