Host cell glucose metabolism during abortive infection by adenovirus type 12

Abstract

Progeny virions were not produced following infection of an established line of African green monkey kidney (Vero) cells with human adenovirus type 12. In addition, viral structural proteins were not synthesized as determined by the immunofluorescent procedure to detect those proteins in infected cells. Cytopathology was observed in the virus-infected Vero cells, but it was less pronounced and differed in some other aspects to the cytopathic effect of adenovirus type 12 during productive infection. Glucose uptake and lactic acid production were monitored as indicators of glucose metabolism over a 48 h period during abortive infection. There was a 50 to 60% increase in glucose uptake and lactic acid production by virus-infected Vero cells compared with parallel cultures of uninfected cells.