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. 1984 Mar 26;12(6):2619-28.
doi: 10.1093/nar/12.6.2619.

Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus

Free PMC article

Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus

K Schmid et al. Nucleic Acids Res. .
Free PMC article

Abstract

Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are (formula: see text) with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages lambda and phi X174 and virus SV40.

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