Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Dec;80(24):7447-51.
doi: 10.1073/pnas.80.24.7447.

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus

T Takegami, R J Kuhn, C W Anderson, E Wimmer

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus

T Takegami et al. Proc Natl Acad Sci U S A. 1983 Dec.

Abstract

A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.

PubMed Disclaimer

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Virology. 1970 Sep;42(1):100-11 - PubMed
    1. J Virol. 1975 Dec;16(6):1512-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jan;74(1):59-63 - PubMed
    1. Nature. 1977 Jul 21;268(5617):208-13 - PubMed

Publication types