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. 1983 Dec;80(24):7447-51.
doi: 10.1073/pnas.80.24.7447.

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus

T Takegami, R J Kuhn, C W Anderson, E Wimmer

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus

T Takegami et al. Proc Natl Acad Sci U S A. 1983 Dec.

Abstract

A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.

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