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. 1984 Jan;13(1):15-20.
doi: 10.1016/0262-1746(84)90097-0.

Separation and quantitation of leukotrienes by reversed-phase high-performance liquid chromatography

J Verhagen, P Walstra, G A Veldink, J F Vliegenthart, P L Bruynzeel

Separation and quantitation of leukotrienes by reversed-phase high-performance liquid chromatography

J Verhagen et al. Prostaglandins Leukot Med. 1984 Jan.

Abstract

A rapid and sensitive reversed-phase HPLC procedure is reported which allows the simultaneous separation and quantitation of LTC4, 11t-LTC4, LTD4, LTB4, 12epi,6t,8c-LTB4, 6t-LTB4 + 12epi,6t-LTB4, two trihydroxy-eicosatetraenoic acids tentatively identified as 20-OH-LTB4 and 20-OH,12epi,6t,8c-LTB4 and several not yet identified 15-series leukotrienes produced by the cytosol of porcine polymorphonuclear leukocytes.

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