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Comparative Study
. 1984 Jan;11(1):96-8.
doi: 10.1016/0147-619x(84)90012-x.

Physical and functional map of the hemolytic plasmid pSU316

I Andrés, J C Rodriguez, J M Ortiz
Comparative Study

Physical and functional map of the hemolytic plasmid pSU316

I Andrés et al. Plasmid. 1984 Jan.

Abstract

A restriction endonuclease analysis of the hemolytic plasmid pSU316 has allowed location of the cleavage sites for the endonucleases BamHI, XbaI, KpnI, Bg/II, Sa/GI, EcoRI, and HindIII. Hybridization experiments between pSU316 and pED100 have shown that the tra region of pSU316 lies in a segment comprising part of Sa/GI fragments S-1 and S-3 and the entire fragment S-4. The positions of other plasmids coded functions, namely the replication functions and alpha-hemolysin production, have been determined in the physical map.

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