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. 1984 Feb;47(2):436-8.
doi: 10.1128/aem.47.2.436-438.1984.

Use of bacteriophage P1 as a vector for Tn5 insertion mutagenesis

M Quinto, R A Bender

Use of bacteriophage P1 as a vector for Tn5 insertion mutagenesis

M Quinto et al. Appl Environ Microbiol. 1984 Feb.

Abstract

Infection of a strain lysogenic for bacteriophage P1 CM with P1::Tn5 followed by simultaneous selection for the chloroamphenicol resistance associated with the resident prophage and the kanamycin resistance associated with Tn5 results in a large number of independent Tn5 insertion mutations. This superinfection-selection protocol is a fast, easy, and safe way to isolate null mutations in enteric bacteria without generating unwanted cryptic mutations elsewhere in the genome.

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