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. 1984 Feb;3(2):257-62.
doi: 10.1002/j.1460-2075.1984.tb01794.x.

Regulated expression of a gene important for replication of plasmid F in E. coli

L Søgaard-Andersen, L A Rokeach, S Molin

Regulated expression of a gene important for replication of plasmid F in E. coli

L Søgaard-Andersen et al. EMBO J. 1984 Feb.

Abstract

Fusions between the gene encoding the E protein of the IncFI plasmid F and the lac genes were constructed. Analysis of the expression of beta-galactosidase from these fusions shows that the promoter for the E protein gene is located between the incB region and the structural gene for the E protein. Near this promoter is a regulatory site on which a negative control element acts. Most likely the E protein itself acts as a repressor of E gene expression and thus autoregulates its own expression. No other gene products seem to affect the expression of the E protein gene.

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References

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