The nucleotide sequence of fragment HindIII-C of human adenovirus type 5 DNA (map positions 17.1-31.7)

Abstract

This paper describes the sequence of nucleotides 6246-11570 between the two HindIII sites at map positions (m.p.) 17.1 and 31.7 of human adenovirus serotype 5 (Ad5) DNA. It is 99% homologous with the corresponding HindIII-B fragment of adenovirus type 2 (Ad2; Gingeras et al., 1982; Aleström et al., 1982) which has been shown to encode the second, "i", and third leaders of the major late RNAs, the virus-associated RNAs VAI and VAII (all rightward transcripts), and in the opposite sense the early-region E2b mRNAs for a DNA polymerase and for the terminal protein precursor (pTP). Except for the latter, it was possible to determine the coordinates of the corresponding Ad5 RNAs, either by sequence homology with Ad2, or by nuclease S1 analysis. The Ad5 DNA sequence contains the same open reading frames as that of Ad2. The longest of these lie in the r-strand and would specify proteins of Mr 120377 (DNA polymerase) and 74558 (pTP).