Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Apr;81(7):2117-21.
doi: 10.1073/pnas.81.7.2117.

Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol

Y Sugimoto, M Whitman, L C Cantley, R L Erikson

Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol

Y Sugimoto et al. Proc Natl Acad Sci U S A. 1984 Apr.

Abstract

The ability of purified Rous sarcoma virus transforming gene product, pp60v-src, to phosphorylate phosphatidylinositol and diacylglycerol was investigated. Phosphatidylinositol was phosphorylated to form both mono- and diphosphorylated derivatives. 1,2-Diacylglycerol was phosphorylated to form phosphatidic acid. These activities showed the same thermolability and the same sensitivity to inhibitors as shown by the casein kinase activity of pp60v-src. In addition, when serum-starved chicken embryo fibroblasts transformed by a virus mutant temperature-sensitive for transformation were shifted from the nonpermissive to permissive temperature, an increase of 50-100% in the labeling of phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidic acid was observed, as compared to uninfected cells.

PubMed Disclaimer

References

    1. J Lipid Res. 1968 Jul;9(4):532-3 - PubMed
    1. Nature. 1983 Nov 3-9;306(5938):67-9 - PubMed
    1. Virology. 1971 Nov;46(2):470-9 - PubMed
    1. Methods Enzymol. 1974;38:299-308 - PubMed
    1. Cancer Res. 1977 Sep;37(9):2979-84 - PubMed

Publication types

MeSH terms