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. 1984 Apr 1;219(1):149-58.
doi: 10.1042/bj2190149.

Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2

R H Ashley, M J Brammer, R Marchbanks

Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2

R H Ashley et al. Biochem J. .

Abstract

The recently synthesized calcium indicator quin -2 was incorporated into synaptosomes from guinea-pig cerebral cortex following uptake and internal hydrolysis of quin -2 tetra-acetoxymethyl ester. Incubation in physiological media containing 1 mM- or 2 mM-CaCl2 led to equilibrium cytosolic ionized calcium concentrations of 85 +/- 10 nM and 205 +/- 5 nM respectively (mean +/- S.E.M. from eight and eighteen preparations respectively). Cytosolic Ca2+ was elevated following increases in external Ca2+ concentration, plasma membrane depolarization, mitochondrial inhibition, calcium ionophore addition or replacement of external sodium by lithium. Preliminary experiments were performed to assess changes in cytosolic Ca2+ accompanying the release of the neurotransmitter acetylcholine.

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References

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