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. 1984 May 15;782(1):103-5.
doi: 10.1016/0167-4781(84)90111-8.

Preparation of ligation intermediates and related polynucleotide pyrophosphates

B C Chu, L E Orgel

Preparation of ligation intermediates and related polynucleotide pyrophosphates

B C Chu et al. Biochim Biophys Acta. .

Abstract

Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.

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