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. 1982;1(12):1573-8.
doi: 10.1002/j.1460-2075.1982.tb01357.x.

Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen

C Stratowa, J Doehmer, Y Wang, P H Hofschneider

Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen

C Stratowa et al. EMBO J. 1982.

Abstract

A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 -124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v-mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.

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