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. 1984 Jun;158(3):820-5.
doi: 10.1128/jb.158.3.820-825.1984.

Construction and characterization of an Escherichia coli strain with a uncI mutation

N J Gay

Construction and characterization of an Escherichia coli strain with a uncI mutation

N J Gay. J Bacteriol. 1984 Jun.

Abstract

A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombinant plasmid containing the mutation, in a temperature-sensitive polA strain. The mutant strain is phenotypically unc+ but has a reduced growth yield compared to a normal sibling strain.

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