Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jun;158(3):997-1004.
doi: 10.1128/jb.158.3.997-1004.1984.

Physical and genetic analyses of the Inc-I alpha plasmid R64

T Furuichi, T Komano, T Nisioka

Physical and genetic analyses of the Inc-I alpha plasmid R64

T Furuichi et al. J Bacteriol. 1984 Jun.

Abstract

A 126-kilobase (kb) physical and genetic map of the Inc-I alpha plasmid R64 was constructed by using the restriction enzymes, BamHI, SalI, XhoI, HindIII, and EcoRI. The replication (Rep) and incompatability (Inc) functions of this plasmid were located in a 1.75-kb segment of an EcoRI fragment, E10 (3.3 kb). In addition, the genes determining growth inhibition of phage BF23 (Ibf), suppression of dnaG ( Sog ), resistance to tetracycline (Tetr), and resistance to streptomycin ( Strr ) were located on the 5.5-kb HindIII-XhoI fragment, the 8.1-kb EcoRI fragment (E5), the 4.6-kb HindIII fragment (H8), and the 4.1-kb HindIII fragment (H10), respectively. The map of R64 was compared with that of ColIb, which belongs to the Inc-I alpha group.

PubMed Disclaimer

References

    1. Plasmid. 1981 Jan;5(1):53-75 - PubMed
    1. Gene. 1980 Nov;11(3-4):291-8 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13 - PubMed
    1. J Mol Biol. 1982 Jan 15;154(2):181-96 - PubMed
    1. J Bacteriol. 1982 Jul;151(1):1-7 - PubMed

Substances

LinkOut - more resources