Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jun;98(6):2047-54.
doi: 10.1083/jcb.98.6.2047.

Ultrastructural localization of the mannose 6-phosphate receptor in rat liver

H J Geuze, J W Slot, G J Strous, A Hasilik, K Von Figura

Ultrastructural localization of the mannose 6-phosphate receptor in rat liver

H J Geuze et al. J Cell Biol. 1984 Jun.

Abstract

An affinity-purified rabbit antibody against rat liver mannose 6-phosphate receptor (MP-R) was prepared. The antibody was directed against a 215 kd-polypeptide and it recognized both ligand-occupied and free receptor. Anti-MP-R was used for immunofluorescence and immunoelectron microscopy of cryosections from rat liver. MP-R was demonstrated in all parenchymal liver cells, but not in endothelial lining cells. MP-R labeling was found at the entire plasma membrane, in coated pits and coated vesicles, in the compartment of uncoupling receptor and ligand, and in the Golgi complex. Lysosomes showed only scarce MP-R label. In double-labeling immunoelectron microscopy, MP-R co-localized with albumin in the Golgi cisternae and in secretory vesicles with lipoprotein particles. Cathepsin D was associated with MP-R in the Golgi cisternae. This finding indicates that MP-R/cathepsin D complexes traverse the Golgi complex on their way to the lysosomes. The possible involvement of CURL in lysosomal enzyme targeting is discussed.

PubMed Disclaimer

References

    1. J Biol Chem. 1982 Sep 10;257(17):9938-43 - PubMed
    1. Methods Enzymol. 1982;83:392-6 - PubMed
    1. Eur J Biochem. 1982 Jul;125(2):317-21 - PubMed
    1. Eur J Cell Biol. 1982 Jun;27(2):213-20 - PubMed
    1. J Biol Chem. 1982 Oct 25;257(20):12322-31 - PubMed

Publication types

MeSH terms