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. 1984 May;309(5966):327-31.
doi: 10.1038/309327a0.

Kinked DNA in crystalline complex with EcoRI endonuclease

C A Frederick, J Grable, M Melia, C Samudzi, L Jen-Jacobson, B C Wang, P Greene, H W Boyer, J M Rosenberg

Kinked DNA in crystalline complex with EcoRI endonuclease

C A Frederick et al. Nature. 1984 May.

Abstract

The 3 A electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity. The DNA contains a torsional kink and other departures from the B conformation which unwind the DNA and thereby widen the major groove in the recognition site.

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