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. 1984 Apr 25;12(8):3435-44.
doi: 10.1093/nar/12.8.3435.

A colorimetric method for DNA hybridization

M Renz, C Kurz
Free PMC article

A colorimetric method for DNA hybridization

M Renz et al. Nucleic Acids Res. .
Free PMC article

Abstract

A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences complementary to the probe can be visualized directly without the need of tedious cytochemical sandwich methods. This procedure will detect target sequences, a few kilobases long, in the 1- to 5-pg range.

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References

    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. Chromosoma. 1975 Nov 24;53(2):107-17 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 - PubMed
    1. Nature. 1979 Oct 25;281(5733):646-50 - PubMed
    1. Nature. 1979 Dec 6;282(5739):575-9 - PubMed

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