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. 1984 May 11;12(9):3791-806.
doi: 10.1093/nar/12.9.3791.

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli

M A Boss, J H Kenten, C R Wood, J S Emtage
Free PMC article

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli

M A Boss et al. Nucleic Acids Res. .
Free PMC article

Abstract

Genes for a murine mu heavy chain and a lambda light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosome binding site. Induction of transcription from the trp promoter results in accumulation of both light and heavy chain polypeptides in appropriate host strains. Both proteins were found as insoluble products. Following extraction and purification of the immunoglobulin containing fractions, antigen binding activity was recovered. The activity demonstrates essentially the same properties as the antibody from the hybridoma from which the genes were cloned.

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