[Cytomorphological and cytochemical studies on the multiplication of bovine reovirus type 1 in a cell culture]

Abstract

Parallel cytomorphologic and cytochemical studies were carried out on the replication of the bovine reovirus type 1 in the Vero cell line. It was found that a high multiplicity of infection M = 5 the first morphologic changes took place some 12 hours following infection, while at the 24th hour there appeared also the characteristic inclusion bodies. At the same time the appearance was noted of acidophile masses in the cytoplasm of some of the infected cells. The morphologic changes reached the peak values of their development at the 48th hour, and the cytoplasmic inclusions assumed a net-shaped structure of lower density. It was also found that following a short-term rise in the first 12 hours after infection there set in a general suppression of the RNA-containing material, which spread irregularly through-out the cell thus marking the nonpyroninophile viral inclusions. The desoxiribonucleic synthesis was not visibly affected, however, the nuclear chromatin remained with an unchanged structure. The viral inclusions were Feulgen PAS-negative. The hydrolytic enzymes alkaline and acid phosphatase and 5-nucleotidase were strongly inhibited after a short-term rise within the first 12 hours of infection.