Comparative Study
Gene transfer into mammalian cells
- PMID: 6329857
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Comparative Study
Gene transfer into mammalian cells
Dev Biol Stand.
1983.
Abstract
The introduction of purified genes into animal cells allows the study of gene expression and regulation and the establishment of genetically modified cell lines. Several methods are available of transferring DNA into cells of higher eukaryotes: transfection with calcium phosphate, fusion of cells with liposomes or bacterial protoplasts, and micro-injection of DNA. Transient and/or permanent expression of foreign genes can be obtained in biochemically transformed cells.
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