doi: 10.1139/g76-068.
Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole
- PMID: 63310
- DOI: 10.1139/g76-068
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Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole
Can J Genet Cytol.
1976 Sep.
Abstract
Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.
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