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. 1976 Sep;18(3):545-7.
doi: 10.1139/g76-068.

Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole

M S Lin, O S Alfi, G N Donnell

Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole

M S Lin et al. Can J Genet Cytol. 1976 Sep.

Abstract

Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.

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