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. 1984 Jun;47(6):1337-40.
doi: 10.1128/aem.47.6.1337-1340.1984.

Ghost cells as sorption matrix for virus concentration from water

R Armon, Y Kott, I Neeman

Ghost cells as sorption matrix for virus concentration from water

R Armon et al. Appl Environ Microbiol. 1984 Jun.

Abstract

Pig erythrocyte membranes were used as adsorbent material for the concentration of bacteriophage phi x-174, MS-2, and f2 from 5 ml of saline solution. The adsorption was carried out at pH 3.5, and the elution was carried out at pH 7.0. Compared with adsorption on 3% beef extract, bacteriophage adsorption on erythrocyte ghost cells yielded 93 to 100%, and elution was 92 to 100%, whereas beef extract organic flocculation yielded adsorption of 0 to 98.8% and elution of 61 to 86.6%. The same procedure but with poliovirus LSC-1 gave 100% adsorption and 91 to 129% elution.

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References

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