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. 1984 Oct 1;223(1):271-4.
doi: 10.1042/bj2230271.

The active site of the P99 beta-lactamase from Enterobacter cloacae

B Joris, J Dusart, J M Frere, J van Beeumen, E L Emanuel, S Petursson, J Gagnon, S G Waley

The active site of the P99 beta-lactamase from Enterobacter cloacae

B Joris et al. Biochem J. .

Abstract

Labelling the beta-lactamase of Enterobacter cloacae P99 with a poor substrate or a mechanism-based inactivator points to an active-site serine residue in a sequence closely resembling that of the ampC beta-lactamase. These results establish the P99 enzyme as a class-C beta-lactamase, and the concurrence of the two approaches helps to confirm the reliability of determining active-site sequences with the aid of mechanism-based inactivators.

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