Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Oct 1;223(1):271-4.
doi: 10.1042/bj2230271.

The active site of the P99 beta-lactamase from Enterobacter cloacae

B Joris, J Dusart, J M Frere, J van Beeumen, E L Emanuel, S Petursson, J Gagnon, S G Waley

The active site of the P99 beta-lactamase from Enterobacter cloacae

B Joris et al. Biochem J. .

Abstract

Labelling the beta-lactamase of Enterobacter cloacae P99 with a poor substrate or a mechanism-based inactivator points to an active-site serine residue in a sequence closely resembling that of the ampC beta-lactamase. These results establish the P99 enzyme as a class-C beta-lactamase, and the concurrence of the two approaches helps to confirm the reliability of determining active-site sequences with the aid of mechanism-based inactivators.

PubMed Disclaimer

References

    1. J Mol Biol. 1970 Sep 14;52(2):165-78 - PubMed
    1. Methods Enzymol. 1975;43:678-87 - PubMed
    1. Methods Enzymol. 1977;47:51-69 - PubMed
    1. Biochem J. 1979 Jan 1;177(1):365-7 - PubMed
    1. Biochemistry. 1980 Jun 24;19(13):2895-901 - PubMed

Publication types