Effects of digestion with N-oligosaccharide glycopeptidase upon certain lectin-peroxidase-diaminobenzidine reactions of glycoproteins in mammalian and avian tissues

Abstract

To determine the histochemical localization of asparagine-linked oligosaccharides of glycoproteins in a series of different mammalian and avian tissues, the effects of digestion with N-oligosaccharide glycopeptidase upon certain lectin-peroxidase-diaminobenzidine reactions of the histological structures involved have been studied by light microscopy. Throughout the tissues examined, asparagine-linked oligosaccharides of glycoproteins were localized mainly in histological structures of connective and muscular tissues, but were hardly or not visualized in those of epithelial tissues. These results appear to lead to the concept that connective and muscular tissues represent the main sites where plasma types of glycoproteins are involved in mammalian and avian species.