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. 1983 Jan;47(1):135-45.
doi: 10.1038/bjc.1983.16.

Human hybridomas from patients with malignant disease

K Sikora, T Alderson, J Ellis, J Phillips, J Watson
Free PMC article

Human hybridomas from patients with malignant disease

K Sikora et al. Br J Cancer. 1983 Jan.
Free PMC article

Abstract

Lymphocytes from 180 patients with a variety of malignant diseases were collected and fused with a human myeloma-derived line, LON-LICR-HMy2/CAM1. A total of 162 hybridomas was obtained. Only B lymphocyte markers were found on the surface of the fusion products. Flow cytometric analysis revealed a stably increased DNA content in the hybridoma cells. Some hybridoma supernatants were found to contain new Ig chains. Anti-tumour binding activity was found in 12 supernatants.

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