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. 1983 Feb;153(2):1083-7.
doi: 10.1128/jb.153.2.1083-1087.1983.

Q beta replicase containing a Bacillus stearothermophilus elongation factor

L Stringfellow, T Blumenthal

Q beta replicase containing a Bacillus stearothermophilus elongation factor

L Stringfellow et al. J Bacteriol. 1983 Feb.

Abstract

We purified Q beta replicase containing EF-Ts from Bacillus stearothermophilus in place of the homologous polypeptide from Escherichia coli. The hybrid enzyme was fully active in the transcription of a variety of templates. It was found to be qualitatively similar to native Q beta replicase with respect to a variety of parameters which measure the efficiency of initiation of RNA synthesis. The results demonstrated that Q beta replicase can tolerate substantial alterations in the EF-Tu X Ts component of the enzyme. These alterations resulted in only minor perturbations of catalytic properties.

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