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. 1983 Jan;80(1):1-5.
doi: 10.1073/pnas.80.1.1.

Expression of hepatitis B surface antigen gene in yeast

A Miyanohara, A Toh-e, C Nozaki, F Hamada, N Ohtomo, K Matsubara

Expression of hepatitis B surface antigen gene in yeast

A Miyanohara et al. Proc Natl Acad Sci U S A. 1983 Jan.

Abstract

The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 X 10(5) molecules per cell of immunologically active HBsAg polypeptide in phosphate-free medium. The HBsAg polypeptides produced in the yeast cells were assembled into 20- to 22-nm spherical or oval particles and were immunogenic.

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