Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Jan;80(1):85-9.
doi: 10.1073/pnas.80.1.85.

Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans

M D Meglasson, P T Burch, D K Berner, H Najafi, A P Vogin, F M Matschinsky

Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans

M D Meglasson et al. Proc Natl Acad Sci U S A. 1983 Jan.

Abstract

Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. The enzyme eluted in two separate peaks. Sigmoidal rate dependence was found with respect to glucose (Hill coefficient = 1.5) for both enzyme fractions. Km values for glucose were 5.7 mM for the major fraction and 4.5 mM for the minor fraction. Neither fraction phosphorylated GlcNAc. A GlcNAc kinase (ATP:2-acetamido-2-deoxy-D-glucose 6-phosphotransferase, EC 2.7.1.59)-enriched fraction, prepared by affinity chromatography on Sepharose-N-(6-aminohexanoyl)-GlcNAc, had a Km of 25 microM for GlcNAc. Islet tissue also contained hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) eluting in multiple peaks. The results are consistent with the concept that glucokinase serves as the glucose sensor of pancreatic beta cells.

PubMed Disclaimer

References

    1. Biochem J. 1976 Oct 1;159(1):7-14 - PubMed
    1. Curr Top Cell Regul. 1976;11:1-50 - PubMed
    1. Endocrinology. 1977 Jan;100(1):1-8 - PubMed
    1. Endocrinology. 1977 May;100(5):1276-83 - PubMed
    1. Biochem J. 1977 Jul 1;165(1):61-9 - PubMed

Publication types

LinkOut - more resources