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. 1983 Jan 25;11(2):265-75.
doi: 10.1093/nar/11.2.265.

Amplification of ribonuclease II (rnb) activity in Escherichia coli K-12

W P Donovan, S R Kushner
Free PMC article

Amplification of ribonuclease II (rnb) activity in Escherichia coli K-12

W P Donovan et al. Nucleic Acids Res. .
Free PMC article

Abstract

A 7.1 kb HindIII-XhoI fragment of E. coli DNA which contains the structural gene for ribonuclease II (rnb) has been cloned in the recombinant plasmid pDK24. At least two constitutively expressed genes are encoded on the fragment as shown by maxicell analysis. On denaturing polyacrylamide gels RNase II appears as a single 72,000 dalton species. The approximate site of transcription initiation of the rnb gene has been mapped. Although derivatives of E. coli harboring pDK24 contained 10-fold more RNase II activity that wild type strains without the plasmid, the degradation rate of mRNA was similar in all strains tested. Strains deficient in both RNase II and polynucleotide phosphorylase appear inviable.

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