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. 1983 Feb;80(3):697-701.
doi: 10.1073/pnas.80.3.697.

Gene for staphylococcal protein A

S Löfdahl, B Guss, M Uhlén, L Philipson, M Lindberg

Gene for staphylococcal protein A

S Löfdahl et al. Proc Natl Acad Sci U S A. 1983 Feb.

Abstract

The gene for protein A from Staphylococcus aureus was cloned into pBR322 in Escherichia coli. An immunoassay was used to detect production of the protein. Protein A produced in E. coli was found in the periplasmic space and was purified and concentrated by IgG-Sepharose affinity chromatography. DNA sequence assay of the gene revealed a region with the general features of a prokaryotic signal peptide and a fifth structural region homologous to the four repetitive regions found earlier by amino acid sequence determination of the mature protein. Upstream from the structural gene there is a possible promoter region and a ribosomal binding sequence typical of gram-positive bacteria. The initiation codon is TTG.

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References

    1. J Biol Chem. 1965 Sep;240(9):3685-92 - PubMed
    1. J Immunol. 1966 Dec;97(6):822-7 - PubMed
    1. Science. 1967 Jun 16;156(3781):1451-5 - PubMed
    1. J Mol Biol. 1969 May 14;41(3):459-72 - PubMed
    1. JAMA. 1971 Dec 13;218(11):1671-3 - PubMed

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