Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D
- PMID: 6341083
- DOI: 10.1016/0014-5793(83)80469-4
Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D
Abstract
A heat-stable enterotoxin produced by a strain of enterotoxigenic Escherichia coli 18D was purified by ion-exchange and reversed-phase high-pressure liquid chromatography. The amino acid sequence of the purified toxin was determined by Edman-degradation and a combination of fast atom bombardment mass spectrometry and carboxypeptidase digestion to be Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr.
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