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. 1983 Jan 1;209(1):151-7.
doi: 10.1042/bj2090151.

Purification and properties of tryptophan synthase from baker's yeast (Saccharomyces cerevisiae)

C J Bailey, P D Turner

Purification and properties of tryptophan synthase from baker's yeast (Saccharomyces cerevisiae)

C J Bailey et al. Biochem J. .

Abstract

Tryptophan synthase was purified from baker's yeast. The purified enzyme exhibited one band on polyacrylamide-gel electrophoresis, had no detectable N-terminal amino acid and C-terminal alanine. The amino acid composition was close to that predicted by recent studies on the DNA sequence of the structural gene for the enzyme. Kinetic parameters for the following three activities were measured: indole-serine condensation, indolylglycerol phosphate lyase and the overall reaction of serine with 1-(indol-3-yl)glycerol 3-phosphate. The Km for indole was much lower than suggested by previous investigations, and the value of 11 microM was measured by a fluorimetric assay.

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