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. 1983 Jan 1;209(1):265-7.
doi: 10.1042/bj2090265.

Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein

H Khalfan, R Abuknesha, D Robinson

Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein

H Khalfan et al. Biochem J. .

Abstract

A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Anal Biochem. 1972 Apr;46(2):598-603 - PubMed
    1. Anal Biochem. 1982 Apr;121(2):290-4 - PubMed
    1. J Steroid Biochem. 1979 Nov-Dec;11(5-6):1597-1600 - PubMed
    1. Annu Rev Biochem. 1980;49:63-91 - PubMed