Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein
- PMID: 6342607
- PMCID: PMC1154083
- DOI: 10.1042/bj2090265
Fluorigenic method for the assay of proteinase activity with the use of 4-methylumbelliferyl-casein
Abstract
A method for the preparation of casein labelled with the 4-methylumbelliferyl fluorophore is described, and the product was used as a fluorigenic macromolecular substrate for a sensitive assay of the activity of proteinases. Nanogram quantities of trypsin, chymotrypsin, elastase and cathepsin D can be detected, but the substrate is unaffected by cathepsin B.
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