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. 1983 Apr 15;145(1):209-17.
doi: 10.1016/s0014-4827(83)80022-6.

The timing of the S phase and other nuclear events in yeast meiosis

D H Williamson, L H Johnston, D J Fennell, G Simchen

The timing of the S phase and other nuclear events in yeast meiosis

D H Williamson et al. Exp Cell Res. .

Abstract

The length of the premeiotic S phase in individual cells of a homothallic strain of Saccharomyces cerevisiae was determined by pulse-labelling synchronously sporulating cultures with [3H]adenine and following changes in the frequency of cells in S by DNA-specific whole cell autoradiography. The average S phase was found to be at least 2-3 times as long as in mitotic diploids, and it was concluded that activation of replication origins in meiosis is considerably staggered. The timing of S in relation to other meiotic milestones was established by counting different nuclear morphologies visualised by DAPI-fluorescent staining. This allowed tentative identification of pachytene nuclei as well as first and second nuclear divisions.

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