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. 1983 May 11;11(9):2617-26.
doi: 10.1093/nar/11.9.2617.

The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region

S Tabata, A Oka, K Sugimoto, M Takanami, S Yasuda, Y Hirota
Free PMC article

The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region

S Tabata et al. Nucleic Acids Res. .
Free PMC article

Abstract

The replication origin of the E. coli K-12 chromosome has been isolated as autonomously replicating molecules(oriC plasmid), and the DNA region essential for replicating function(oriC) has been localized to a sequence of 232-245 base-pairs(bp) by deletion analysis. In this report, the functional role of oriC was analysed by using an in vitro replication system and various OriC+ and OriC- plasmids previously constructed. The results obtained were summarized as follows: (1) The oriC sequence contained information enough to direct bidirectional replication. (2) The actual DNA replication began at a region near, but outside, oriC and progressed bidirectionally. (3) Initiation of DNA synthesis at the specific region required the dnaA-complementing fraction from cells harboring a dnaA-carrying plasmid.

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