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. 1983 Jun 24;740(2):163-8.
doi: 10.1016/0167-4781(83)90073-8.

Occurrence of histone H10-related protein fraction in trout liver

Occurrence of histone H10-related protein fraction in trout liver

L Srebreva et al. Biochim Biophys Acta. .

Abstract

The lysine-rich histones of trout liver were studied in order to see whether a protein similar to mammalian histone H1(0) exists in this lower vertebrate. The biochemical methods used involved SDS and acid-urea polyacrylamide gel electrophoresis, gel exclusion and ion-exchange chromatography. Specific rabbit antibodies were elicited against chromatographically-purified mouse liver H1(0) and applied for further characterization of the presumptive trout-liver H1(0) fraction. The results from the indirect immunofluorescence on nitrocellulose-immobilized crude H1 subfractions and from the complement fixation test supplied additional evidence for the existence of an H1(0)-like protein in trout liver.

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